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KMID : 0811720030070000192
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Korean Journal of Physiology & Pharmacology
2003 Volume.7 No. 0 p.192 ~ p.0
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Cockroach Extract Induces Intracellular Ca2+ Oscillations from Thapsigargin-sensitive Ca2+ Stores in Human Airway Epithelial Cells
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Hong Jeong-Hee
Lee Syng-Ill
Seo Jeong-Taeg
Shin Dong-Min
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Abstract
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The German cockroach has been reported to act as an allergen, which may be partly associated with a protease reaction in asthma. However, the molecular identity of the antigens in German cockroach extract (GCE) has with active protease activity, and the protease-activated receptors (PARs) that interact with is activated by GCE in human airway epithelial cells have not been characterized. We investigated the direct effects of GCE on Ca2+ signaling in human airway epithelial cells in terms of Ca2+ signaling, and the type of PAR activated by GCE. involved in generating intracellular Ca2+ signaling, and further identified the source of the Ca2+ mobilization. GCE induced a baseline type of [Ca2+]i oscillations in a dose-dependent manner. These were found to be The oscillations independent of persisted for long periods of time in the absence of Ca2+ entry across the plasma membrane, suggesting that the observed [Ca2+]i increases were due to Ca2+ release from intracellular Ca2+ stores. Accordingly, after depleting Ca2+ in the endoplasmic recticulum (ER) Ca2+ with thapsigargin, an ER- Ca2+ ATPase inhibitor, in the absence of extracellular Ca2+, the GCE-mediated did not increase [Ca2+]i signals were abolished., and in the presence of GCE thapsigargin did not increase [Ca2+]i. Whereas desensitization of PAR-1, 3, and 4 had no effect on GCE-mediated Ca2+ mobilization, Whereas GCE mobilized Ca2+ after the desensitization of PAR-1, 3, and 4 with repeated treatments of thrombin, no GCE-mediated [Ca2+]i increases were increase was observed with GCE challenge after desensitization with trypsin, an activator of PAR-2. These results indicate that GCE has a direct effect on human airway epithelial cells, in particular, generating [Ca2+]i oscillations by Ca2+ release from thapsigargin- sensitive Ca2+ stores via through activation of PAR-2.
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KEYWORD
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German cockroach, Ca2+ signaling, Protease-activated receptor, Allergen, Human airway epithelial cell
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